Journal: Journal of Hematology & Oncology

Article Title: Cell adhesion-mediated mitochondria transfer contributes to mesenchymal stem cell-induced chemoresistance on T cell acute lymphoblastic leukemia cells

doi: 10.1186/s13045-018-0554-z

Figure Lengend Snippet: Increased expression of ICAM-1 in MSCs facilitates cell adhesion and protects Jurkat cells from chemotherapeutic drugs. a Confocal top view and side view of Jurkat cells (prestained with MitoTracker Red) and GFP-labeled MSCs in a coculture system. Scale bar, 10 μm. b mRNA expression of adhesion molecules in MSCs cocultured with Jurkat cells. c Representative photos of Jurkat cells and MSCs in the coculture system after removal of the nonadhesive Jurkat cells. Scale bar, 50 μm. d The relative adhesion ratio was calculated as the ratio of the number of Jurkat cells adhered to MSCs in the anti-ICAM-1 treated group to that in the DMSO-treated group. e The death rate of Jurkat cells was examined by FACS. f A CCK-8 assay was used to assess Jurkat cell viability. The data above are presented as the mean ± S.E.M. of three independent experiments (* P < 0.05; ** P < 0.01; t test)

Article Snippet: Neutralizing anti-ICAM-1 antibody (MS305PABX) was purchased from Invitrogen (Carlsbad, CA, USA).

Techniques: Expressing, Labeling, CCK-8 Assay

Journal: Journal of Hematology & Oncology

Article Title: Cell adhesion-mediated mitochondria transfer contributes to mesenchymal stem cell-induced chemoresistance on T cell acute lymphoblastic leukemia cells

doi: 10.1186/s13045-018-0554-z

Figure Lengend Snippet: Cell-cell adhesion mediates mitochondria transfer from Jurkat cells to MSCs. Anti-ICAM-1 (20 mg/ml) was added to the coculture system with MTX for 48 h. a Confocal images showed that mitochondria transfer was inhibited by anti-ICAM-1. Scale bar, 10 μm. b The areas of red foci per field were calculated by ImageJ software. c Flow cytometry analysis of MitoTracker Red uptake by MSCs (GFP+ population) cocultured with MitoTracker Red-labeled Jurkat cells for 48 h. d The percentage of Red+ MSCs in each group was analyzed and graphed. The data above are presented as the mean ± S.E.M. of three independent experiments (* P < 0.05; ** P < 0.01; t test)

Article Snippet: Neutralizing anti-ICAM-1 antibody (MS305PABX) was purchased from Invitrogen (Carlsbad, CA, USA).

Techniques: Software, Flow Cytometry, Labeling